• Product NameCyclin D1 (Phospho-Thr286) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 286 (A-C-T(p)-P-T) derived from Human Cyclin D1.
• Target NameCyclin D1
• ModificationPhospho
• Alternative Names    BCL-1 ; oncogene; CYL-1; PRAD1; PRAD1
• Accession No. Swiss-Prot#: P24385;
  NCBI Gene#: 595;
  NCBI Protein#: NP_444284.1
• UniprotP24385
• Gene ID595;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40
Tagliati F, et al. (2006) Endocrinology 147, 3530-8
Zou Y, et al. (2004) J Biol Chem 279, 27790-8

• Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.
• Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500
• Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:233

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting