• Product NameCyclin D1 (Phospho-Ser90) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Cyclin D1 only when phosphorylated at serine 90.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 90(F-L-S(p)-L-E) derived from Human Cyclin D1 .
• Target NameCyclin D1
• ModificationPhospho
• Alternative Names    BCL1; CCND1; CYL-1; PRAD1; PRAD1 oncogene
• Accession No. Swiss-Prot#: P24385;
  NCBI Gene#: 595;
  NCBI Protein#: NP_444284.1
• UniprotP24385
• Gene ID595;
• SDS-PAGE MW 31kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Sewing A, M¨¹ller R (1994) Oncogene 9, 2733-6

• Western blot analysis of extracts from K562 tissue using Cyclin D1 (Phospho-Ser90) antibody #11986.The lane on the right is treated with the antigen-specific peptide.
• Western Blot analysis of lysates of MCF7 treated with LPS and MCF7, using primary antibody at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting