• Product NameCD45 (Phospho-Ser1007) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of CD45 only when phosphorylated at serine 1007.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 1007 (D-D-S(p)-D-S) derived from Human CD45.
• Target NameCD45
• ModificationPhospho
• Alternative Names    L-CA; LY-5; Leukocyte common antigen precursor; PTPRC; T200
• Accession No. Swiss-Prot#: P08575;
  NCBI Gene#: 5788;
  NCBI Protein#: NP_001254727.1
• UniprotP08575
• Gene ID5788;
• SDS-PAGE MW 150kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding.

Wang Y, Guo W, Liang L, Esselman WJ (1999)J Biol Chem 274, 7454-61.

• Western blot analysis of extracts from HeLa cells treated with TNF using Phospho-CD45 (Ser1007) antibody #11993.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting