• Product NameBax (Phospho-Thr167) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of Bax only when phosphorylated at threonine 167.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine167 (P-G-T(p)-P-T) derived from Human Bax.
• Target NameBax
• ModificationPhospho
• Alternative Names    BAXA; Apoptosis regulator BAX; membrane isoform alpha; Bcl2-associated X protein;
• Accession No. Swiss-Prot#: Q07812;
  NCBI Gene#: 581;
  NCBI Protein#: NP_620116.1.
• UniprotQ07812
• Gene ID581;
• SDS-PAGE MW 21kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunofluorescence: 1:100~1:200

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Francesca Andriani. J Natl Cancer Inst, Sep 2001; 93: 1314 - 1324.
H Martinez-Valdez. J. Exp. Med., Mar 1996; 183: 971.
Joslyn K. Brunelle. J. Biol. Chem., Feb 2004; 279: 4305 - 4312.

• Immunofluorescence staining of methanol-fixed A549 cells using Bax (Phospho-Thr167) Antibody #11680.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting