• Product NamePKA-R2β (Phospho-Ser114) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of PKA-R2β only when phosphorylated at serine 114.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine114(R-A-S(p)-V-C) derived from Human PKA-R2β.
• Target NamePKA-R2β
• ModificationPhospho
• Alternative Names    KAP3; PRKAR2B; PKA R2-beta;
• Accession No. Swiss-Prot#: P31323;
  NCBI Gene#: 5577;
  NCBI Protein#: NP_002727.2.
• UniprotP31323
• Gene ID5577;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Levy F.O., Mol. Endocrinol. 2:1364-1373(1988).
DeGiorgis J.A., J. Proteome Res. 4:306-315(2005).
Han G., Proteomics 8:1346-1361(2008)

• Western blot analysis of extracts from COS-7 cells treated with PMA using PKA-R2β (Phospho-Ser114) Antibody #11692.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting