• Product NamehnRPD (Phospho-Ser83) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD .
• Target NamehnRPD
• ModificationPhospho
• Alternative Names    ROD; AUF1; HNRPD;
• Accession No. Swiss-Prot#: Q14103;
  NCBI Gene#: 3184;
  NCBI Protein#: NP_112738.1.
• UniprotQ14103
• Gene ID3184;
• SDS-PAGE MW 38kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Kajita Y., J. Biol. Chem. 270:22167-22175(1995).
Dempsey L.A., Genomics 49:378-384(1998).
Tolnay M., Biochem. J. 338:417-425(1999).
Grosset C., Cell 103:29-40(2000)

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody #11696 (left)or the same antibody preincubated with blocking peptide (right).
• Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody #11696.
• Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody #11696. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting