• Product NameEzrin(Ab-567) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Ezrin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 565~569 (Y-K-T-L-R) derived from Human Ezrin.
• Target NameEzrin
• Alternative Names    Cytovillin; EZRI; VIL2; Villin 2; p81
• Accession No. Swiss-Prot: P15311
  NCBI Protein: NP_001104547.1
• UniprotP15311
• Gene ID7430;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 81kd
  Western blotting: 1:500~1:1000
  

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91.
Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95.
Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90.
Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9.

• Western blot analysis of extracts from Hela,MDA and 3T3 cells using Ezrin(Ab-567) Antibody #21200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting