• Product NameSNAI1 (Phospho-Ser246) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of SNAI1 only when phosphorylated at serine 246.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 246(T-F-S(p)-R-M) derived from Human SNAI1.
• Target NameSNAI1
• ModificationPhospho
• Alternative Names    SNAH; SNAI; Sna; Snail;
• Accession No. Swiss-Prot#: O95863;
  NCBI Gene#: 6615;
  NCBI Protein#: NP_005976.2.
• UniprotO95863
• Gene ID6615;
• SDS-PAGE MW 29kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.

Okubo T., Cancer Res. 61:1338-1346(2001).
Twigg S.R., Hum. Genet. 105:320-326(1999).
Paznekas W.A., Genomics 62:42-49(1999).

• Western blot analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody #11709.The lane on the right is treated with the antigen-specific peptide.
• Immunofluorescence staining of methanol-fixed HuvEc cells using SNAI1 (Phospho-Ser246) Antibody #11709.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting