• Product NameHP1α (Phospho-Ser92) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of HP1α only when phosphorylated at serine 92.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 92(R-S-S(p)-N-F) derived from Human HP1α .
• Target NameHP1α
• ModificationPhospho
• Alternative Names    HP1A; HP1 alpha; antigen p25;
• Accession No. Swiss-Prot#: P45973;
  NCBI Gene#: 23468;
  NCBI Protein#: NP_001120793.1.
• UniprotP45973
• Gene ID23468;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.

Saunders W.S., J. Cell Sci. 104:573-582(1993).
Ye Q., J. Biol. Chem. 271:14653-14656(1996).
Ye Q., J. Biol. Chem. 272:14983-14989(1997).

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody #11777 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting