• Product NameB-Raf (Phospho-Thr753) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 753 (P-K-T(p)-P-L) derived from Human B-Raf.
• Target NameB-Raf
• ModificationPhospho
• Alternative Names    B-RAF; RAFB1; RMIL; p94;
• Accession No. Swiss-Prot#: P15056;
  NCBI Gene#: 673;
  NCBI Protein#: NP_004324.2.
• UniprotP15056
• Gene ID673;
• SDS-PAGE MW 84kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992).
Albert S., Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W., Science 300:767-772(2003)

• Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody #11786.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting