• Product NameCtBP1 (Phospho-Ser422) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of CtBP1 only when phosphorylated at serine 422.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 422(A-P-S(p)-P-G) derived from Human CtBP1.
• Target NameCtBP1
• ModificationPhospho
• Alternative Names    CTBP; C-terminal binding protein 1; EC 1.1.1;
• Accession No. Swiss-Prot#: Q13363;
  NCBI Gene#: 1487;
  NCBI Protein#: NP_001319.1.
• UniprotQ13363
• Gene ID1487;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Schaeper U., Proc. Natl. Acad. Sci. U.S.A. 92:10467-10471(1995).
Sewalt R.G.A.B., Mol. Cell. Biol. 19:777-787(1999).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody #11796.The lane on the right is treated with the antigen-specific peptide.
• Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody #11796 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting