• Product NameEPS15 (Phospho-Tyr849) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of EPS15 only when phosphorylated at tyrosine 849.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15.
• Target NameEPS15
• ModificationPhospho
• Alternative Names    AF1P; MLLT5; EPS15; Protein Eps15;
• Accession No. Swiss-Prot#: P42566;
  NCBI Gene#: 2060;
  NCBI Protein#: NP_001972.1.
• UniprotP42566
• Gene ID2060;
• SDS-PAGE MW 100kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Wong W.T., Oncogene 9:1591-1597(1994).
Bernard O.A., Oncogene 9:1039-1045(1994).

• Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody #11800. The lane on the left is treated with antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting