• Product NameCNOT2 (Phospho-Ser101) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of CNOT2 only when phosphorylated at serine 101.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine101(S-L-S(p)-Q-G) derived from Human CNOT2.
• Target NameCNOT2
• ModificationPhospho
• Alternative Names    NOT2H; CDC36; CNOT2; HSPC131;
• Accession No. Swiss-Prot#: Q9NZN8;
  NCBI Gene#: 4848;
  NCBI Protein#: NP_001186231.1.
• UniprotQ9NZN8
• Gene ID4848;
• SDS-PAGE MW 59kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Immunohistochemistry: 1:50~1:100

The CCR4-NOT complex functions as general transcription regulation complex. Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. 5 isoforms of the human protein are produced by alternative splicing.

Albert T.K., Nucleic Acids Res. 28:809-817(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).
Ota T., Nat. Genet. 36:40-45(2004).

• Immunohistochemical analysis of paraffin-embedded human brain tissue using CNOT2 (Phospho-Ser101) antibody #11810 (left)or the same antibody preincubated with blocking peptide (right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting