• Product NameHK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total HK1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human HK1 (NP_000179.2).
• Target NameHK1
• Alternative Names    HK1;HK;HK1-ta;HK1-tb;HK1-tc;HKD;HKI;HMSNR;HXK1;hexokinase;RP79
• Accession No. Uniprot:P19367
  GeneID:3098
• UniprotP19367
• Gene ID3098
• SDS-PAGE MW 102KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

• Immunohistochemistry of paraffin-embedded rat kidney using HK1 Rabbit pAb.
• Immunofluorescence analysis of A-549 cells using [KO Validated] HK1 Rabbit pAb.
• Immunofluorescence analysis of C6 cells using [KO Validated] HK1 Rabbit pAb.
• Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] HK1 Rabbit pAb.
• Immunohistochemistry of paraffin-embedded mouse kidney using HK1 Rabbit pAb.
• Western blot analysis of extracts of various cell lines, using HK1 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting