• Product NameCAPN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total CAPN1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human Calpain 1 (NP_005177.2).
• Target NameCAPN1
• Alternative Names    CAPN1;CANP;CANP1;CANPL1;SPG76;muCANP;muCL;calpain 1
• Accession No. Uniprot:P07384
  GeneID:823
• UniprotP07384
• Gene ID823
• SDS-PAGE MW 75-80KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using Calpain 1 antibody.
• Immunohistochemistry of paraffin-embedded rat brain using Calpain 1 Rabbit pAb.
• Immunofluorescence analysis of NIH/3T3 cells using Calpain 1 antibody.
• Immunofluorescence analysis of HeLa cells using Calpain 1 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting