• Product NameSPARC Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total SPARC protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human SPARC (NP_003109.1).
• Target NameSPARC
• Alternative Names    SPARC;BM-40;OI17
• Accession No. Uniprot:P09486
  GeneID:6678
• UniprotP09486
• Gene ID6678
• SDS-PAGE MW 35kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

• Immunohistochemistry of paraffin-embedded rat Intestine using SPARC antibody.
• Western blot analysis of extracts of various cell lines, using SPARC antibody.
• Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibody.
• Immunohistochemistry of paraffin-embedded human lung cancer using SPARC antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting