• Product NameRFC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total RFC1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human RFC1 (NP_001191676.1).
• Target NameRFC1
• Alternative Names    RFC1;A1;MHCBFB;PO-GA;RECC1;RFC;RFC140
• Accession No. Uniprot:P35251
  GeneID:5981
• UniprotP35251
• Gene ID5981
• SDS-PAGE MW 140KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

• Western blot analysis of extracts of HeLa cells, using RFC1 antibody.
• Western blot analysis of extracts of various cell lines, using RFC1 antibody.
• Immunofluorescence analysis of A549 cells using RFC1 antibody.
• Immunofluorescence analysis of MCF-7 cells using RFC1 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting