• Product NameBUB1B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total BUB1B protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human BUB1B (NP_001202.4).
• Target NameBUB1B
• Alternative Names    BUB1B;BUB1beta;BUBR1;Bub1A;MAD3L;MVA1;SSK1;hBUBR1
• Accession No. Uniprot:O60566
  GeneID:701
• UniprotO60566
• Gene ID701
• SDS-PAGE MW 55kDa/120kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

• Immunofluorescence analysis of NIH-3T3 cells using BUB1B antibody.
• Western blot analysis of extracts of various cell lines, using BUB1B antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting