• Product NameDCK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total DCK protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human DCK.
• Target NameDCK
• Alternative Names    MGC117410; MGC138632;
• Accession No. Swiss-Prot:P27707
  NCBI Gene ID:1633
• UniprotP27707
• Gene ID1633;
• SDS-PAGE MW 31KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443). This antibody is specific to DCK.

• Western blot analysis of extracts of various cell lines, using DCK at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat kidney using DCK at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human gastric cancer using DCK at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using DCK . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting