• Product NameCD47 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total CD47 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human CD47.
• Target NameCD47
• Alternative Names    IAP; OA3; MER6;
• Accession No. Swiss-Prot:Q08722
  NCBI Gene ID:961
• UniprotQ08722
• Gene ID961;
• SDS-PAGE MW 35KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.

• Western blot analysis of extracts of various cell lines, using CD47 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human lung cancer using CD47 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human placenta using CD47 at dilution of 1:200 (40x lens).
• Immunofluorescence analysis of L929 cells using CD47 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using CD47 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting