• Product NamePOLI Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total POLI protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human POLI.
• Target NamePOLI
• Alternative Names    RAD30B; RAD3OB;
• Accession No. Swiss-Prot:Q9UNA4
  NCBI Gene ID:11201
• UniprotQ9UNA4
• Gene ID11201;
• SDS-PAGE MW 80KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

Mammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates ¡°G¡± opposite template ¡°T¡± more frequently than incorporates the correct ¡°A.¡± It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. . It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress.

• Western blot analysis of extracts of various cell lines, using POLI at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting