• Product NameKPNA4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total KPNA4 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human KPNA4.
• Target NameKPNA4
• Alternative Names    QIP1; SRP3; IPOA3;
• Accession No. Swiss-Prot:O00629
  NCBI Gene ID:3840
• UniprotO00629
• Gene ID3840;
• SDS-PAGE MW 57KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

• Immunofluorescence analysis of U2OS cells using KPNA4 . Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat heart using KPNA4 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 at dilution of 1:200 (40x lens).
• Western blot analysis of extracts of various cell lines, using KPNA4 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting