• Product NameCaMKII(Ab-286) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total CaMKII protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.284~288 (Q-E-T-V-D) derived from Human CaMKII.
• Target NameCaMKII
• Alternative Names    CAMKA
• Accession No. Swiss-Prot: Q9UQM7
  NCBI Protein: NP_057065.2
• UniprotQ9UQM7
• Gene ID815;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 50kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity

J Bossuyt, K Helmstadter, X Wu, et al. (2008) Ca2+/Calmodulin-Dependent Protein Kinase II {delta} and Protein Kinase D Overexpression Reinforce - Circulation Research, 102(6):695-702.
This article references the use of the #21279.

• Western blot analysis of extracts from Hela using CaMKII(Ab-286) Antibody #21279.
• Immunofluorescence staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using CaMKII(Ab-286) Antibody #21279.
• Western blotting analysis using CaMKII(Ab-286) Antibody #21279.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting