• Product NameHSP90B(Ab-254) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total HSP90B protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 252~256 (V-G-S-D-E) derived from Human HSP90B.
• Target NameHSP90B
• Alternative Names    HSP 84
• Accession No. Swiss-Prot: P08238
  NCBI Protein: NP_031381.2
• UniprotP08238
• Gene ID3326;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 92kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Molecular chaperone. Has ATPase activity.

Ingrid K. Ruf, et,al. (2005) J. Virol ; 79: 14562 - 14569.
Christy M. Hebner,et,al. (2006) J. Gen. Virol ; 87: 3183 - 3193.
Rika van Huizen, et,al. (2003) J. Biol. Chem ; 278: 15558 - 15564.

• Western blot analysis of extracts from Hela and A2780 cells using HSP90B(Ab-254) Antibody #21290.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B(Ab-254) Antibody #21290(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP90B(Ab-254) Antibody #21290.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting