• Product NamePLK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PLK1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human PLK1 (NP_005021.2).
• Target NamePLK1
• Alternative Names    PLK1;PLK;STPK13
• Accession No. Uniprot:P53350
  GeneID:5347
• UniprotP53350
• Gene ID5347
• SDS-PAGE MW 62KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.

• Immunohistochemistry of paraffin-embedded human colon carcinoma using PLK1 Rabbit pAb.
• Western blot analysis of extracts of various cell lines, using PLK1 antibody.
• Immunofluorescence analysis of C6 cells using PLK1 Rabbit pAb.
• Immunofluorescence analysis of NIH-3T3 cells using PLK1 Rabbit pAb.
• Immunofluorescence analysis of U-2 OS cells using PLK1 Rabbit pAb.
• Immunohistochemistry of paraffin-embedded human placenta using PLK1 Rabbit pAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting