• Product NameEIF4A1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeIgG
• PurificationAffinity-chromatography
• ApplicationsWB;ICC/IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total EIF4A1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionA synthesized peptide derived from human eIF4A1
• Target NameEIF4A1
• Alternative Names    EIF4A1;DDX2A;EIF-4A;EIF4A;eIF-4A-I;eIF4A-I
• Accession No. Uniprot:P60842
  GeneID:1973
• UniprotP60842
• Gene ID1973
• SDS-PAGE MW 45KDa
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:2000;
  ICC/IF 1:50-1:200

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

• Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb.
• Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb.
• Immunofluorescence analysis of NIH/3T3 cells using eIF4A1 Rabbit pAb.
• Western blot analysis of eIF4A1 expression in MCF7 cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting