• Product NameCUL2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total CUL2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human CUL2.
• Target NameCUL2
• Alternative Names    Cullin-2;
• Accession No. Swiss-Prot:Q13617
  NCBI Gene ID:8453
• UniprotQ13617
• Gene ID8453;
• SDS-PAGE MW 87KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

CUL2 is a core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. It serves as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

• Western blot analysis of extracts of various cell lines, using CUL2 at 1:1000 dilution.
• Immunofluorescence analysis of A-549 cells using CUL2 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting