• Product NameGTF2A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total GTF2A1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human GTF2A1 (NP_056943.1).
• Target NameGTF2A1
• Alternative Names    GTF2A1;TF2A1;TFIIA;TFIIA-42;TFIIAL
• Accession No. Uniprot:P52655
  GeneID:2957
• UniprotP52655
• Gene ID2957
• SDS-PAGE MW 37kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]

• Western blot analysis of extracts of various cell lines, using GTF2A1 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting