• Product NameASH2L Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total ASH2L protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ASH2L.
• Target NameASH2L
• Alternative Names    ASH2; Bre2; ASH2L1; ASH2L2;
• Accession No. Swiss-Prot:Q9UBL3
  NCBI Gene ID:9070
• UniprotQ9UBL3
• Gene ID9070;
• SDS-PAGE MW 69KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:20 - 1:50

Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.

• Western blot analysis of extracts of various cell lines, using ASH2L at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using ASH2L . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting