• Product NameMCM5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total MCM5 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MCM5.
• Target NameMCM5
• Alternative Names    CDC46; P1-CDC46;
• Accession No. Swiss-Prot:P33992
  NCBI Gene ID:4174
• UniprotP33992
• Gene ID4174;
• SDS-PAGE MW 82KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:1000 - 1:3000
  IF 1:50 - 1:200

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

• Western blot analysis of extracts of various cell lines, using MCM5 at 1:3000 dilution.
• Immunofluorescence analysis of U2OS cells using MCM5 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting