• Product NameMAP1LC3A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total MAP1LC3A protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MAP1LC3A.
• Target NameMAP1LC3A
• Alternative Names    LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Accession No. Swiss-Prot:Q9H492
  NCBI Gene ID:84557
• UniprotQ9H492
• Gene ID84557;
• SDS-PAGE MW 14KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  Immunohistochemistry: 1:50 - 1:200
  Immunofluorescence: 1:50 - 1:200

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

• Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody at 1:500 dilution.
• Immunofluorescence analysis of HeLa cells using LC3A/LC3B antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting