• Product NameMECOM Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total MECOM protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MECOM.
• Target NameMECOM
• Alternative Names    EVI1; MDS1; PRDM3; MDS1-EVI1; AML1-EVI-1
• Accession No. Swiss-Prot:Q13465
  NCBI Gene ID:2122
• UniprotQ03112
• Gene ID2122;
• SDS-PAGE MW 18KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  Immunohistochemistry: 1:50 - 1:200

The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using MECOM antibody.
• Immunohistochemistry of paraffin-embedded rat pancreas using MECOM antibody at dilution of 1:200 (400x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting