• Product NameLC3a Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total LC3A protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.
• Target NameLC3a
• Alternative Names    Microtubule-associated protein 1 light chain 3 alpha; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1 light chain 3-like protein 1; Autophagy-related protein LC3 A
• Accession No. Swiss-Prot: Q9H492
  NCBI Protein: NP_115903.1
• UniprotQ9H492
• Gene ID84557;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 18 16 kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Probably involved in formation of autophagosomal vacuoles (autophagosomes).

• Western blot analysis of extracts from Hela and 293 cells using LC3A antibody #21402.
• Immunofluorescence staining of methanol-fixed Hela cells using LC3A antibody #21402.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting