• Product NameNCOA4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total NCOA4 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human NCOA4.
• Target NameNCOA4
• Alternative Names    RFG; ELE1; PTC3; ARA70;
• Accession No. Swiss-Prot:Q13772
  NCBI Gene ID:8031
• UniprotQ13772
• Gene ID8031;
• SDS-PAGE MW 69KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:200

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.

• Immunohistochemistry of paraffin-embedded human lung cancer using NCOA4 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using NCOA4 at 1:1000 dilution.
• Western blot analysis of extracts from normal (control) and NCOA4 knockout (KO) HeLa cells, using NCOA4 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting