• Product NameHPSE Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total HPSE protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human HPSE.
• Target NameHPSE
• Alternative Names    HPA; HPA1; HPR1; HSE1; HPSE1
• Accession No. Swiss-Prot:Q9Y251
  NCBI Gene ID:10855
• UniprotQ9Y251
• Gene ID10855;
• SDS-PAGE MW 61KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:10 - 1:100

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using Heparanase 1 at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using Heparanase 1 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting