• Product NamePLCg1(Ab-771) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PLC-g1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 769~773 (P-D-Y-G-A) derived from Human PLC-g1.
• Target NamePLCg1
• Alternative Names    Phosphoinositide phospholipase C; Phospholipase C-gamma-1;
• Accession No. Swiss-Prot: P19174
  NCBI Protein: NP_002651.2
• UniprotP19174
• Gene ID5335;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 155kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

• Western blot analysis of extracts from 3T3 cells using PLC-g1(Ab-771) Antibody #21523.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-g1(Ab-771) Antibody #21523(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting