• Product Namec-kit(Ab-936) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total c-Kit protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.934~938 (H-I-Y-S-N) derived from Human c-Kit.
• Target Namec-kit
• Alternative Names    KIT; CD117; SCFR; PBT;
• Accession No. Swiss-Prot: P10721
  NCBI Protein: NP_000213.1
• UniprotP10721
• Gene ID3815;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 145kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Martin, F.H. et al. (1990) Cell 63, 203-11.
Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
Nocka, K. et al. (1990) EMBO J 9, 1805-13.

• Western blot analysis of extracts from HL60 cells using c-kit(Ab-936) Antibody #21539.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit(Ab-936) Antibody #21539(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting