• Product NameFra-2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Fra-2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Fra-2.
• Target NameFra-2
• Alternative Names    FLJ23306; FOS-RELATED ANTIGEN 2;
• Accession No. Swiss-Prot: P15408
  NCBI Gene ID: 2355
• UniprotP15408
• Gene ID2355;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Controls osteoclast survival and size By similarity. As a dimer with JUN, activates LIF transcription By similarity. Activates CEBPB transcription in PGE2-activated osteoblasts By similarity.

Masao Murakami, Cell Growth Differ., May 1999; 10: 333.
Martin Smith, Mol. Cell. Biol., Jun 2001; 21: 3704 - 3713.
L. K. Mccauley, Endocrinology, May 2001; 142: 1975.
Tetsuo Yoshida, Nucleic Acids Res., Jun 1993; 21: 2715 - 2721.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Fra-2 antibody #33377.
• Western blot analysis of extracts from LOVO cells, using Fra-2 antibody #33377.
• Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using Fra-2 antiobdy #33377. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting