• Product NameMet(Ab-1003) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Met protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 1001~1005 (V-D-Y-R-A) derived from Human Met.
• Target NameMet
• Alternative Names    Scatter factor receptor
• Accession No. Swiss-Prot: P08581
  NCBI Protein: NP_000236.2
• UniprotP08581
• Gene ID4233;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 156kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.

Weidner, K.M. et al. (1993) J. Cell Biol. 121, 145-154.
Traxler, P. et al. (2001) Med. Res. Rev. 21, 499-512.
Schaeper, U. et al. (2000) J. Cell Biol. 149, 1419-1432.
Park, M. et al. (1986) Cell 45, 895-904.

• Western blot analysis of extracts from A431 and HepG2 cells using Met(Ab-1003) Antibody #21548.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Met(Ab-1003) Antibody #21548(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting