• Product NameCD40 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CD40 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human CD40.
• Target NameCD40
• Alternative Names    B-cell surface antigen CD40; Bp50; CD40 antigen; CD40L receptor; CDw40
• Accession No. Swiss-Prot: P25942
  NCBI Gene ID: 958
• UniprotP25942
• Gene ID958;
• SDS-PAGE MW 30kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

Carlos Reyes-Moreno, J. Biol. Chem., May 2007; 10.1074.
Hongwei Qin, Blood, Nov 2005; 106: 3114 - 3122.
Meera J. Nathan, J. Immunol., Jun 2004; 172: 6626 - 6633.
Clare C. Davies, Mol. Cell. Biol., Nov 2005; 25: 9806 - 9819.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CD40 antibody #33507.
• Western blot analysis of extracts from COS7 cells, using CD40 antibody #33507.
• Western blot analysis of extracts from JK cells (Lane 2), K562 cells (Lane 3) and 293 cells (Lane 4), using CD40 antiobdy #33507. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting