• Product NameBCKD Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total BCKD protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human BCKD.
• Target NameBCKD
• Alternative Names    [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial [Precursor]; EC 2.7.11.4; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKDHKIN
• Accession No. Swiss-Prot: O14874
  NCBI Gene ID: 10295
• UniprotO14874
• Gene ID10295;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex By similarity.

Chuang J.C., Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from 293 cells and Jurkat cells, using BCKD antibody #33570.
• Immunofluorescence analysis of HeLa cells, using BCKD antibody #33570.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting