• Product NameAF10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total AF10 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human AF10.
• Target NameAF10
• Alternative Names    Protein AF-10; MLLT10; AF10;
• Accession No. Swiss-Prot: P55197
  NCBI Gene ID: 8028
• UniprotP55197
• Gene ID8028;
• SDS-PAGE MW 109kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.

Chaplin T., Blood 85:1435-1441(1995).
Deloukas P., Nature 429:375-381(2004).
Matsuoka S., Science 316:1160-1166(2007).

• Western blot analysis of extracts from COLO205 cells and Jurkat cells, using AF10 antibody #33820.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting