• Product NameIRX3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total IRX3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human IRX3.
• Target NameIRX3
• Alternative Names    iroquois homeobox 3; IRX-1;
• Accession No. Swiss-Prot: P78415
  NCBI Gene ID: 79191
• UniprotP78415
• Gene ID79191;
• SDS-PAGE MW 52kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  

Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells By similarity.

Hansen L., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Lewis M.T., Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IRX3 antibody #33836.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting