• Product NameFAK(Phospho-Tyr397) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 397(D-D-Y(p)-A-E)derived from Human FAK.
• Target NameFAK
• ModificationPhospho
• Alternative Names    FAK; FAK1; PTK2
• Accession No. Swiss-Prot: Q05397
  NCBI Protein: NP_001186578.1
• UniprotQ05397
• Gene ID5747;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 125kd
  Western blotting: 1:500~1:1000
   Immunohistochemistry: 1:50 - 1:100 

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Matsuya M.et.al. (1998)J. Biol. Chem. 273:1003-1014
Fujita H.et.al. (1998)J. Biol. Chem. 273:26516-26521
Le Romancer M.et.al. (2008)Mol. Cell 31:212-221

• Western blot analysis of extract from 293 cells untreated or treated with EGF using FAK(Phospho-Tyr397) Antibody using #11215
• Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting