• Product NameCHKB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CHKB protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CHKB.
• Target NameCHKB
• Alternative Names    Choline/ethanolamine kinase; Choline kinase beta (EC 2.7.1.32)(CK); Ethanolamine kinase (EC 2.7.1.82)(EK); CHKB; CHETK
• Accession No. Swiss-Prot: Q9Y259
  NCBI Gene ID: 1120
• UniprotQ9Y259
• Gene ID1120;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis.

Yamazaki N., J. Biol. Chem. 275:31739-31746(2000).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Yu L.-R., J. Proteome Res. 6:4150-4162(2007).

• Western blot analysis of extracts from HepG2 cells and A549 cells, using CHKB antibody #34125.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting