• Product NameH-NUC Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total H-NUC protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human H-NUC.
• Target NameH-NUC
• Alternative Names    CDC27Hs; Cell division cycle protein 27 homolog; H-NUC;
• Accession No. Swiss-Prot: P30260
  NCBI Gene ID: 996
• UniprotP30260
• Gene ID996;
• SDS-PAGE MW 92kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Tugendreich S., Proc. Natl. Acad. Sci. U.S.A. 90:10031-10035(1993).
Chen P.L., Cell Growth Differ. 6:199-210(1995).
Kraft C., EMBO J. 22:6598-6609(2003).

• Western blot analysis of extracts from HUVEC cells, HepG2 cells and COLO205 cells, using H-NUC antibody #34190.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting