• Product NameTubulin b-III Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total Tubulin b-III protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.442~446(E-E-S-E-A) derived from Human Tubulin b-III.
• Target NameTubulin b-III
• Alternative Names    TUBB4; CFEOM3A; TUBB3
• Accession No. Swiss-Prot: Q13509
  NCBI Protein: NP_006077.2
• UniprotQ13509
• Gene ID10381;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 55kd
  Western blotting: 1:500~1:1000
  

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the a-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Tischfield M.A., Baris H.N., Wu C., Rudolph G. Cell 140:74-87(2010)
Katsetos C.D., Legido A., Perentes E., Mork S.J. J. Child Neurol. 18:851-866(2003)

• Western blot analysis of extract from Mouse brain tissue, Hela, JK and P19 cells using Tubulin beta-III Antibody #21617

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting