• Product NameATG16L1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ATG16L1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human ATG16L1.
• Target NameATG16L1
• Alternative Names    A16L1; APG16 autophagy 16-like; APG16-like 1; APG16L; APG16L beta
• Accession No. Swiss-Prot: Q676U5
  NCBI Gene ID: 55054
• UniprotQ676U5
• Gene ID55054;
• SDS-PAGE MW 68kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Zheng H., DNA Seq. 15:303-305(2004).
Hampe J., Nat. Genet. 39:207-211(2007).
Clark H.F., Genome Res. 13:2265-2270(2003).

• Western blot analysis of extracts from Jurkat cells, using ATG16L1 antibody #34474.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting