• Product NameRab5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Rab5 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.188~192( N-P-G-A-N) derived from Human Rab5.
• Target NameRab5
• Alternative Names    RAB5A
• Accession No. Swiss-Prot: P20339
  NCBI Protein: NP_004153.2
• UniprotP20339
• Gene ID5868;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 25kd
  Western blotting: 1:500~1:1000
  

Required for the fusion of plasma membranes and early endosomes.

Callaghan J.M., Nixon S., Bucci C., Toh B.-H., Stenmark H.Eur. J. Biochem. 265:361-366(1999) Hoffenberg S., Liu X., Nikolova L., Hall H.S., Dai W., Baughn R.E., Dickey B.F., Barbieri M.A., Aballay A., Stahl P.D., Knoll B.J.J. Biol. Chem. 275:24661-24669(2000)

• Western blot analysis of extract from Hela, MCF and PC12 cells using Rab5 Antibody #21628

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting