• Product NameSPARC Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total SPARC protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
• Target NameSPARC
• Alternative Names    ON; BM-40;
• Accession No. Swiss-Prot: P09486
  NCBI Protein: NP_003109.1
• UniprotP09486
• Gene ID6678;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 42kd
  Western blotting: 1:500~1:1000
  

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)
Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988)
Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)

• Western blot analysis of extract from T98G cells using SPARC Antibody #21633

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting